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Proceedings Paper

Two-photon excited fluorescence lifetime imaging microscopy for FRET study on protein interactions
Author(s): Junle Qu; Ziyang Lin; Lixin Liu; Xuan Guo; Danni Chen; Hanben Niu
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Paper Abstract

Two-photon excited fluorescence lifetime imaging (2P-FLIM) provides a more direct and precise approach to fluorescence resonance energy transfer (FRET), which allows studying the dynamic behavior of protein-protein interactions in living cells. In this paper, we describe the combination of a Leica TCS SP2 laser scanning microscope and a time-correlated single photon counting (TCSPC) lifetime imaging module developed by Becker & Hickl for two-photon excited fluorescence lifetime imaging. This 2P-FLIM system was used for FRET study on the interaction of heat shock protein hsp27 with p38 MAP kinase in the single living cell. Results show that the reduction in donor (CFP) lifetime in the presence of acceptor (YFP) reveals interactions between the two proteins.

Paper Details

Date Published: 18 January 2005
PDF: 6 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.569582
Show Author Affiliations
Junle Qu, Shenzhen Univ. (China)
Ziyang Lin, Shenzhen Univ. (China)
Lixin Liu, Shenzhen Univ. (China)
Xuan Guo, Shenzhen Univ. (China)
Danni Chen, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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