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Proceedings Paper

Cell-based bioassays in microfluidic systems
Author(s): Laura J. Itle; Jeanna C. Zguris; Michael V. Pishko
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Paper Abstract

The development of cell-based bioassays for high throughput drug screening or the sensing of biotoxins is contingent on the development of whole cell sensors for specific changes in intracellular conditions and the integration of those systems into sample delivery devices. Here we show the feasibility of using a 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate, a fluorescent dye capable of responding to changes in intracellular pH, as a detection method for the bacterial endotoxin, lipopolysaccharide. We used photolithography to entrap cells with this dye within poly(ethylene) glyocol diacrylate hydrogels in microfluidic channels. After 18 hours of exposure to lipopolysaccharide, we were able to see visible changes in the fluorescent pattern. This work shows the feasibility of using whole cell based biosensors within microfluidic networks to detect cellular changes in response to exogenous agents.

Paper Details

Date Published: 7 December 2004
PDF: 10 pages
Proc. SPIE 5588, Smart Medical and Biomedical Sensor Technology II, (7 December 2004); doi: 10.1117/12.569094
Show Author Affiliations
Laura J. Itle, The Pennsylvania State Univ. (United States)
Jeanna C. Zguris, The Pennsylvania State Univ. (United States)
Michael V. Pishko, The Pennsylvania State Univ. (United States)


Published in SPIE Proceedings Vol. 5588:
Smart Medical and Biomedical Sensor Technology II
Brian M. Cullum, Editor(s)

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