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Proceedings Paper

Development of a multispectral multiphoton fluorescence lifetime imaging microscopy system using a streak camera
Author(s): Junle Qu; Lixin Liu; Baoping Guo; Ziyang Lin; Tao Hu; Jindong Tian; Shuyan Wang; Jikang Zhang; Hanben Niu
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Paper Abstract

We report on the development of a multispectral multiphoton fluorescence lifetime imaging microscopy (MM-FLIM) system that is the combination a streak camera, a prism spectrophotometer, a femtosecond Ti: Sapphire laser and a fluorescence microscope. This system is versatile with multispectral capability, high temporal (10ps) and spatial (0.36μm) resolution and can be used to make 3-dimensional (3D) (x-y-z) multiphoton fluorescence intensity, spectrally resolved intensity and lifetime measurements with a single detector. The system was calibrated with a F-P etalon and a standard fluorescent dye and the lifetime value obtained was in good agreement with the value reported in the literature. Preliminary results suggest that this MM-FLIM system has integrated high temporal, spatial, and spectral resolution fluorescence detection in one microscopy system. Potential applications of this system include multiwell imaging, tissue discrimination, intracellular physiology and fluorescence resonance energy transfer imaging.

Paper Details

Date Published: 18 January 2005
PDF: 7 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.568736
Show Author Affiliations
Junle Qu, Shenzhen Univ. (China)
Lixin Liu, Shenzhen Univ. (China)
Xi'an Institute of Optics and Precision Mechanics, CAS (China)
Baoping Guo, Shenzhen Univ. (China)
Ziyang Lin, Shenzhen Univ. (China)
Tao Hu, Shenzhen Univ. (China)
Jindong Tian, Shenzhen Univ. (China)
Shuyan Wang, Shenzhen Univ. (China)
Jikang Zhang, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)


Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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