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Proceedings Paper

Structure of insoluble immune complexes as studied by spectroturbidimetry and dynamic light scattering
Author(s): Boris N. Khlebtsov; Gennadii L. Burygin; Larisa Yu. Matora; Sergei Yu. Shchyogolev; Nikolai G. Khlebtsov
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Paper Abstract

We describe two variants of a method for determining the average composition of insoluble immune complex particles (IICP). The first variant is based on measuring the specific turbidity (the turbidity per unit mass concentration of the dispersed substance) and the average size of IICP determined from dynamic light scattering (DLS). In the second variant, the wavelength exponent (i.e., the slope of the logarithmic turbidity spectrum) is used in combination with specific turbidity measurements. Both variants allow the average biopolymer volume fraction to be determined in terms of the average refractive index of IICP. The method is exemplified by two experimental antigen+antibody systems: (i) lipopolysaccharide-protein complex (LPPC) of Azospirillum brasilense Sp245+rabbit anti-LPPC; and (ii) human IgG (hIgG)+sheep anti-hIgG. Our measurements by the two methods for both types of systems gave, on the average, the same result: the volume fraction of the IICP biopolymers is about 30%; accordingly, the volume fraction of buffer solvent is 70%.

Paper Details

Date Published: 14 July 2004
PDF: 12 pages
Proc. SPIE 5475, Saratov Fall Meeting 2003: Coherent Optics of Ordered and Random Media IV, (14 July 2004); doi: 10.1117/12.568567
Show Author Affiliations
Boris N. Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russia)
Gennadii L. Burygin, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russia)
Larisa Yu. Matora, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russia)
Sergei Yu. Shchyogolev, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russia)
Saratov State Univ. (Russia)
Nikolai G. Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russia)
Saratov State Univ. (Russia)


Published in SPIE Proceedings Vol. 5475:
Saratov Fall Meeting 2003: Coherent Optics of Ordered and Random Media IV
Dmitry A. Zimnyakov, Editor(s)

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