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Proceedings Paper

A novel UV-laser source for fluorescence excitation of proteins
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Paper Abstract

A novel, compact and robust UV laser has been developed for laser induced fluorescence spectroscopy of biomolecules in the spectral region from 290 nm to 345 nm. It was based on a frequency-doubled passively Q-switched Nd:YAG laser, emitting at 532 nm, which was pumping a periodically poled KTiOPO4 optical parametric oscillator with intra-cavity sum-frequency mixing in a BBO crystal. The output was generated in two branches in the UV, 293 nm and 343 nm, with pulse widths of 1.8 ns and pulse repetition rate of 100 Hz. These wavelengths were then used for fluorescence experiments of bioagents.

Paper Details

Date Published: 29 December 2004
PDF: 8 pages
Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); doi: 10.1117/12.568436
Show Author Affiliations
Mikael Tiihonen, Royal Institute of Technology (Sweden)
Valdas Pasiskevicius, Royal Institute of Technology (Sweden)
Fredrik Laurell, Royal Institute of Technology (Sweden)
Mikael Lindgren, Norwegian Univ. of Science and Technology (Norway)


Published in SPIE Proceedings Vol. 5617:
Optically Based Biological and Chemical Sensing for Defence
John C. Carrano; Arturas Zukauskas, Editor(s)

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