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Proceedings Paper

Immobilized DNA biosensor based on evanescent wave long-period fiber gratings
Author(s): Jian Yang; Chang-Qing Xu; Razvan Nutiu; Yingfu Li
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Paper Abstract

A DNA aptamer sensor using a long-period grating (LPG) is proposed for the sensitive detection of the interaction between the DNA aptamer and its target. The DNA aptamer can be immobilized on the surface of the LPG, and the detection of the specific interaction between the DNA aptamer and its target is monitored optically. This sensor operates on the evanescent field interacting with the DNA aptamer immobilized over the grating region. The reaction between the DNA aptamer and its target alter the refractive index and thus change the LPG transmission spectrum, which can be monitored in real time. This technology eliminates the labeling of the DNA aptamer. In this paper, for the first time, the dependence of the LPG transmission spectrum on the characteristic of the LPG and the property of the immobilized DNA aptamer are studied both theoretically and experimentally. Experimental demonstration of the immobilization of DNA aptamer on LPG and its real-time effect on the transmission spectrum are reported.

Paper Details

Date Published: 9 December 2004
PDF: 8 pages
Proc. SPIE 5578, Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education, (9 December 2004); doi: 10.1117/12.567502
Show Author Affiliations
Jian Yang, McMaster Univ. (Canada)
Chang-Qing Xu, McMaster Univ. (Canada)
Razvan Nutiu, McMaster Univ. (Canada)
Yingfu Li, McMaster Univ. (Canada)


Published in SPIE Proceedings Vol. 5578:
Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education
Marc Nantel; Glen Herriot; Graham H. McKinnon; Leonard MacEachern; Robert A. Weersink; Rejean Munger; Andrew Ridsdale, Editor(s)

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