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Proceedings Paper

In vitro cytotoxicity assessment of [5,10,15,20-tetra (4-sulfophenyl) porphyrin] on tumor and nontumor cell lines
Author(s): R. Alexandrova; O. Sabotinov; Elena V. Stoykova; Rodica-Mariana Ion; Stanislav Shurulinkov; Georgi Minchev
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Paper Abstract

In this study we evaluate the cytotoxicity of 5,10,15,20- tetra (4-sulfophenyl) porphyrins on a tumor cell line LSCC-SF-Mc29, obtained from a transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29, a timor line LSR-SF-SR, obtained from a transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and for normal mouse cell line (BALB/c-3T3-A31) and bovine kidney cell line (MDBK). The cells were exposed to irradiation from a pulsed CuBr vapor laser system at 510.6 nm and 578.2 nm at fluence rate 50 mW/cm2 and pulse frequency rate 20 kHz. The viability of cells was determined by the neutral red uptake cytotoxicity assay. The light dose-response curves and light exposures that ensure viability drop to 50 % were obtained for each cell line. The cytotoxic effect of TS4PP is most distinguished for LSCC-SF-Mc29. The bovine cell line is more vulnerable than the mouse line, especially at 510.6 nm. The 2-4 times higher viability of the normal cell lines in comparison with the tumor lines has been obtained.

Paper Details

Date Published: 21 June 2004
PDF: 8 pages
Proc. SPIE 5449, Eighth International Conference on Laser and Laser Information Technologies, (21 June 2004); doi: 10.1117/12.563137
Show Author Affiliations
R. Alexandrova, Institute of Experimental Pathology and Parasitology (Bulgaria)
O. Sabotinov, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Elena V. Stoykova, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Rodica-Mariana Ion, Institute for Chemical Research (Romania)
Stanislav Shurulinkov, Central Lab. of Optical Storage and Processing of Information (Bulgaria)
Georgi Minchev, Central Lab. of Optical Storage and Processing of Information (Bulgaria)


Published in SPIE Proceedings Vol. 5449:
Eighth International Conference on Laser and Laser Information Technologies
Vladislav Ya. Panchenko; Nikola V. Sabotinov, Editor(s)

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