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Proceedings Paper

FTIR spectroscopy, site-directed mutagenesis, and isotope labeling: a new approach for studying membrane proteins
Author(s): Yi-Wu He; Mark P. Krebs; Judy Herzfeld; H. Gobind Khorana; Kenneth J. Rothschild
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Paper Abstract

The FTIR absorption spectrum of a membrane protein contains bands due to the vibrations of individual chemical groups. Site-directed mutagenesis can be used to assign such bands to individual amino acid residues. Using this method, a band from the amino acid residue Tyr- 185 of bacteriorhodopsin, a light-driven proton pump, was identified in the low temperature FTIR absorption spectrum. Its intensity and frequency indicate that Tyr-185 exists in a partially deprotonated form in light-adapted bacteriorhodopsin.

Paper Details

Date Published: 1 March 1992
PDF: 8 pages
Proc. SPIE 1575, 8th Intl Conf on Fourier Transform Spectroscopy, (1 March 1992); doi: 10.1117/12.56283
Show Author Affiliations
Yi-Wu He, Boston Univ. (United States)
Mark P. Krebs, Massachusetts Institute of Technology (United States)
Judy Herzfeld, Brandeis Univ. (United States)
H. Gobind Khorana, Massachusetts Institute of Technology (United States)
Kenneth J. Rothschild, Boston Univ. (United States)


Published in SPIE Proceedings Vol. 1575:
8th Intl Conf on Fourier Transform Spectroscopy

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