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Proceedings Paper

Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections
Author(s): Bjarni Bodvarsson; Soren Klim; Stig Mortensen; Martin Morkebjerg; James Chen; Julian R. Maclaren; Chun Hong Yoon; Pradeep K. Luther; John M. Squire; Andrew Bainbridge-Smith; Philip J. Bones; Rick P. Millane
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Paper Abstract

An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined using h-dome extraction and image filtering, based on grayscale reconstruction. Erroneous locations are eliminated based on lattice regularity. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and classified using a Gaussian mixture model. Application to a number of micrographs and comparison with manual classifications of orientations shows that the system is effective in many cases.

Paper Details

Date Published: 22 October 2004
PDF: 12 pages
Proc. SPIE 5562, Image Reconstruction from Incomplete Data III, (22 October 2004); doi: 10.1117/12.562357
Show Author Affiliations
Bjarni Bodvarsson, Univ. of Canterbury (New Zealand)
Soren Klim, Univ. of Canterbury (New Zealand)
Stig Mortensen, Univ. of Canterbury (New Zealand)
Martin Morkebjerg, Univ. of Canterbury (New Zealand)
James Chen, Univ. of Canterbury (New Zealand)
Julian R. Maclaren, Univ. of Canterbury (New Zealand)
Chun Hong Yoon, Univ. of Canterbury (New Zealand)
Pradeep K. Luther, Imperial College London (United Kingdom)
John M. Squire, Imperial College London (United Kingdom)
Andrew Bainbridge-Smith, Univ. of Canterbury (New Zealand)
Philip J. Bones, Univ. of Canterbury (New Zealand)
Rick P. Millane, Univ. of Canterbury (New Zealand)

Published in SPIE Proceedings Vol. 5562:
Image Reconstruction from Incomplete Data III
Philip J. Bones; Michael A. Fiddy; Rick P. Millane, Editor(s)

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