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Proceedings Paper

Assessment of Nd:YAG laser-induced injury to rabbit nasal septal cartilage using confocal microscopy and Live/Dead assay in an ex vivo model
Author(s): Chao Li; Tatiana B. Krasieva; Roman Zorin; Chung-Ho Sun; Anthony Lam; David M. Gardiner; Brian Jet-Fei Wong
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Paper Abstract

Identification of proliferating chondrocytes along the periphery of laser ablation sites in irradiated cartilage has led to interest in studying the use of laser heating alone to stimulate chondrocyte growth. However, excessive heat produced by a laser can also cause chondrocyte necrosis and apoptosis. The objective of this study was to evaluate acute injury to cartilage following irradiation by an Nd:YAG (λ=1.32μm) laser in intact ex-vivo tissue specimens. Rabbit nasal septal cartilage was irradiated using an Nd:YAG laser using pulse durations (4, 6, and 8 seconds) and power (4, 6, and 8 watts) settings previously determined to produce cell division. Immediately after laser irradiation, the extent of thermal injury to the cartilage samples was evaluated using a Live/Dead cell viability assay combined with confocal microscopy. Thermal injury was assessed with respect to distribution of live and dead cells surrounding the laser spot where regeneration was previously observed. The cell viability assay identified necrotic tissue within and immediately around the laser spot. Moving away from the center of the laser spot, a mixed population of necrotic and live chondrocytes was observed. As expected, a correlation between irradiation time, power and degree of injury was found. The results of this experiment will be used to determine the threshold required to produce regeneration while minimizing thermal injury.

Paper Details

Date Published: 13 July 2004
PDF: 5 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.561729
Show Author Affiliations
Chao Li, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Tatiana B. Krasieva, Beckman Laser Institute (United States)
Roman Zorin, Beckman Laser Institute (United States)
Chung-Ho Sun, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Anthony Lam, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
David M. Gardiner, Univ. of California/Irvine (United States)
Brian Jet-Fei Wong, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)


Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Brian Jet-Fei Wong; Nikiforos Kollias; Kenton W. Gregory; Henry Hirschberg; Reza S. Malek; Abraham Katzir; David S. Robinson; Kenneth Eugene Bartels; Eugene A. Trowers; Werner T.W. de Riese; Lawrence S. Bass; Lloyd P. Tate; Steen J. Madsen; Keith D. Paulsen; Karen M. McNally-Heintzelman, Editor(s)

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