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Proceedings Paper

Simulation of heart infarction by laser microbeams and induction of arrhythmias by optical tweezers
Author(s): Birgit Perner; Shamci Monajembashi; Alexander Rapp; Leo Wollweber; Karl Otto Greulich
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Paper Abstract

Laser microbeam and optical tweezers were used for micromanipulation of a heart tissue model consisting of embryonic chicken cardiomyocytes and bibroblasts. Using the laser microbeam a would was created, i.e. a sort of artificial heart infarction was generated. The first steps of wound repair were observed by live cell imaging. A complete filling of teh would primarily by migrating fibroblasts but not by cardiomyocytes was detected 18 hours after wounding. In another set of experiments erythrocyte mediated force application (EMFA) by optical tweezers was applied for optomechanical manipulatoin of cardiomyocytes and fibroblasts. Here we demonstrate induction of dramatic distrubances of calcium waves in a group of synchronously beating cardiomyocytes by an optomechanical input that results in cellular deformation. Surprisingly, it was found that putatively non-excitable fibroblasts respond to this mechanical stress with calcium oscillations. The results reported here indicate that the induction of artificial heart infarction can provide insights into healing processes after mycardial injury. EMFA is capable to examine effects of myocardial overload and to provide important information about processes triggered by mechanical stress on the level of single or very few cells. As a perspective, the preseneted techniques may be used to study the influence of drugs on wound healing and coordination of beating in the heart.

Paper Details

Date Published: 18 October 2004
PDF: 10 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.560750
Show Author Affiliations
Birgit Perner, Institute for Biomolecular Technology (Germany)
Shamci Monajembashi, Institute for Biomolecular Technology (Germany)
Alexander Rapp, Institute for Biomolecular Technology (Germany)
Leo Wollweber, Institute for Biomolecular Technology (Germany)
Karl Otto Greulich, Institute for Biomolecular Technology (Germany)


Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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