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Proceedings Paper

A practical method to determine the light source distribution in bioluminescent imaging
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Paper Abstract

Optical signatures of tumor cells may be generated by expression of reporter genes encoding bioluminescent/fluorescent proteins. Bioluminescent imaging is a novel technique that identifies such light sources from the light flux detected on the surface of a small animal. This technique can effectively evaluate tumor cell growth and regression in response to various therapies in medical research, drug development and gene therapy. In this paper, the diffusion approximation is employed to describe the propagation of photons through biological tissues. Then, a practical method is proposed for localizing and quantifying bioluminescent sources from external bioluminescent signals. This method incorporates prior knowledge on permissible source regions, and transforms the inverse bioluminescent problem into a finite element-based constrained optimization procedure. This approach is validated and evaluated with ideal and noise data in numerical simulation.

Paper Details

Date Published: 26 October 2004
PDF: 8 pages
Proc. SPIE 5535, Developments in X-Ray Tomography IV, (26 October 2004); doi: 10.1117/12.560524
Show Author Affiliations
Wenxiang Cong, Univ. of Iowa (United States)
Durairaj Kumar, Univ. of Iowa (United States)
Yi Liu, Univ. of Iowa (United States)
Alexander Cong, Univ. of Iowa (United States)
Ge Wang, Univ. of Iowa (United States)

Published in SPIE Proceedings Vol. 5535:
Developments in X-Ray Tomography IV
Ulrich Bonse, Editor(s)

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