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Proceedings Paper

Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence
Author(s): Peng Zhang; Shi-You Ding; Qi Xu; Steve Smith; Garry Rumbles; Michael E. Himmel
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Paper Abstract

We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to self-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the 6x-histidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.

Paper Details

Date Published: 14 October 2004
PDF: 8 pages
Proc. SPIE 5513, Physical Chemistry of Interfaces and Nanomaterials III, (14 October 2004); doi: 10.1117/12.560231
Show Author Affiliations
Peng Zhang, National Renewable Energy Lab. (United States)
Shi-You Ding, National Renewable Energy Lab. (United States)
Qi Xu, National Renewable Energy Lab. (United States)
Steve Smith, National Renewable Energy Lab. (United States)
Garry Rumbles, National Renewable Energy Lab. (United States)
Michael E. Himmel, National Renewable Energy Lab. (United States)


Published in SPIE Proceedings Vol. 5513:
Physical Chemistry of Interfaces and Nanomaterials III
Gregory V. Hartland; Xiao-Yang Zhu, Editor(s)

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