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Proceedings Paper

The formation of blood sediment at low hematocrit: a kinetic study by a laser scattering technique
Author(s): Bronislaw Grzegorzewski; A. Gornicki; S. Czarnecki; A. Gutsze
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Paper Abstract

In this paper the formation of erythrocyte aggregates and their sedimentation was studied by optical method. The movement and aggregation of erythrocytes produce a variation in scattered-light intensity. The well-mixed blood sample at hematocrit of 5% was vertically scanned by laser light. The scattered light intensity was continuously recorded and analyzed. The initially observed increase in the transmitted-light intensity reflected the reorganization of the structure formed by erythrocytes, and was followed by the decrease of light intensity due to an increase of blood sample optical density. In blood samples of 5% hematocrit the new, specific sedimentation curve was obtained. From this curve three different phases of sedimentation process can be distinguished. In the first phase no deposit is observed. This phase corresponds to falling of single cells as well as formation and sedimentation of small aggregates takes place. The second phase, when rapid growth of deposit is observed, corresponds to formation and sedimentation of rouleaux and large aggregates. Finally in the third phase the boundary between deposit and settling cells is slowly going down due to packing of erythrocyte aggregates. These results show that in blood samples of low hematocrit both the kinetics of erythrocyte aggregation and sedimentation process are different from those of the blood with normal and high hematocrit.

Paper Details

Date Published: 4 June 2004
PDF: 8 pages
Proc. SPIE 5477, Sixth International Conference on Correlation Optics, (4 June 2004); doi: 10.1117/12.559832
Show Author Affiliations
Bronislaw Grzegorzewski, Ludwik Rydygier Medical Univ. in Bydgoszcz (Poland)
A. Gornicki, Ludwik Rydygier Medical Univ. in Bydgoszcz (Poland)
S. Czarnecki, Ludwik Rydygier Medical Univ. in Bydgoszcz (Poland)
A. Gutsze, Ludwik Rydygier Medical Univ. in Bydgoszcz (Poland)

Published in SPIE Proceedings Vol. 5477:
Sixth International Conference on Correlation Optics
Oleg V. Angelsky, Editor(s)

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