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Proceedings Paper

Polarization discrimination technique to separate overlapping fluorescence and elastic scattering applied to algae in seawater
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Paper Abstract

We present the results of recent experiments and analysis on the polarization discrimination technique developed by us to separate elastic reflectance and fluorescence components resulting from white light illumination of algae in seawater. The technique uses the polarized properties of elastically scattered light and unpolarized properties of fluorescence to separate the two. The approach was successfully applied to measurements on four types of algae of different sizes and shapes in the laboratory, with both polarized and unpolarized illumination sources, and, with in some cases, the addition of different concentrations of clay as well as in-situ field measurements in eastern Long Island. The procedure is shown to be effective for extraction of the chlorophyll fluorescence in the 685 nm region from reflectance of algae dominated by chlorophyll pigments. We also report the results of preliminary experiments of the impact of surface roughness on the efficacy of the technique for measurements both above and under the surface.

Paper Details

Date Published: 9 November 2004
PDF: 9 pages
Proc. SPIE 5544, Remote Sensing and Modeling of Ecosystems for Sustainability, (9 November 2004); doi: 10.1117/12.559576
Show Author Affiliations
Jing Zhou, CUNY/City College (United States)
Alexander Gilerson, CUNY/City College (United States)
Adrian Gill, CUNY/City College (United States)
Barry M. Gross, CUNY/City College (United States)
Fred Moshary, CUNY/City College (United States)
Samir Ahmed, CUNY/City College (United States)

Published in SPIE Proceedings Vol. 5544:
Remote Sensing and Modeling of Ecosystems for Sustainability
Wei Gao; David R. Shaw, Editor(s)

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