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Proceedings Paper

Application of SERS nanoparticles for intracellular pH measurements
Author(s): Ted Laurence; Chad Talley; Michael Colvin; Thomas R. Huser
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Paper Abstract

We present an alternative approach to optical probes that will ultimately allow us to measure chemical concentrations in microenvironments within cells and tissues. This approach is based on monitoring the surface-enhanced Raman scattering (SERS) response of functionalized metal nanoparticles (50-100 nm in diameter). SERS allows for the sensitive detection of changes in the state of chemical groups attached to individual nanoparticles and small clusters. Here, we present the development of a nanoscale pH meter. The pH response of these nanoprobes is tested in a cell-free medium, measuring the pH of the solution immediately surrounding the nanoparticles. Heterogeneities in the SERS signal, which can result from the formation of small nanoparticle clusters, are characterized using SERS correlation spectroscopy and single particle/cluster SERS spectroscopy. The response of the nanoscale pH meters is tested under a wide range of conditions to approach the complex environment encountered inside living cells and to optimize probe performance.

Paper Details

Date Published: 19 October 2004
PDF: 7 pages
Proc. SPIE 5512, Plasmonics: Metallic Nanostructures and Their Optical Properties II, (19 October 2004); doi: 10.1117/12.559562
Show Author Affiliations
Ted Laurence, Lawrence Livermore National Lab. (United States)
Chad Talley, Lawrence Livermore National Lab. (United States)
Univ. of California/Davis (United States)
Michael Colvin, Lawrence Livermore National Lab. (United States)
Univ. of California/Merced (United States)
Thomas R. Huser, Lawrence Livermore National Lab. (United States)
Univ. of California/Davis (United States)


Published in SPIE Proceedings Vol. 5512:
Plasmonics: Metallic Nanostructures and Their Optical Properties II
Naomi J. Halas; Thomas R. Huser, Editor(s)

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