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Proceedings Paper

Functional micro-imaging of soft and hard tissue using synchrotron light
Author(s): Philipp Johannes Thurner; Peter Wyss; Romain Voide; Martin Stauber; Bert Muller; Marco Stampanoni; Jeffrey A. Hubbell; Ralph Muller; Urs Sennhauser
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Paper Abstract

In current biological and biomedical research, quantitative endpoints have become an important factor of success. Classically, such endpoints were investigated with 2D imaging, which is usually destructive and the 3D character of tissue gets lost. 3D imaging has gained in importance as a tool for both, qualitative and quantitative assessment of biological systems. In this context synchrotron radiation based tomography has become a very effective tool for opaque 3D tissue systems. Cell cultures and adherent scaffolds are visualized in 3D in a hydrated environment, even uncovering the shape of individual cells. Advanced morphometry allows to characterize the differences between the cell cultures of two distinct phenotypes. Moreover, a new device is presented enabling the 3D investigation of trabecular bone under mechanical load in a time-lapsed fashion. Using the highly brilliant X-rays from a synchrotron radiation source, bone microcracks and an indication for un-cracked ligament bridging are uncovered. 3D microcrack analysis proves that the classification of microcracks from 2D images is ambiguous. Fatigued bone was found to fail in burst-like fashion, whereas non-fatigued bone exhibited a distinct failure band. Additionally, a higher increase in microcrack volume was detected in fatigued in comparison to non-fatigued bone. The developed technologies prove to be very effective tools for advanced 3D imaging of both hard and soft tissue.

Paper Details

Date Published: 26 October 2004
PDF: 17 pages
Proc. SPIE 5535, Developments in X-Ray Tomography IV, (26 October 2004); doi: 10.1117/12.559515
Show Author Affiliations
Philipp Johannes Thurner, EMPA Dubendorf (Switzerland)
ETH Zurich (Switzerland)
Peter Wyss, EMPA Dubendorf (Switzerland)
Romain Voide, ETH Zurich (Switzerland)
Martin Stauber, ETH Zurich (Switzerland)
Bert Muller, ETH Zurich (Switzerland)
Marco Stampanoni, ETH Zurich (Switzerland)
Paul Scherrer Institut (Switzerland)
Jeffrey A. Hubbell, Ecole Polytechnique Federale de Lausanne (Switzerland)
Ralph Muller, ETH Zurich (Switzerland)
Urs Sennhauser, EMPA Dubendorf (Switzerland)

Published in SPIE Proceedings Vol. 5535:
Developments in X-Ray Tomography IV
Ulrich Bonse, Editor(s)

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