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Proceedings Paper

Observing the dynamic variation of the binding force between rhodostomin ligand and integrin alpha(IIb)beta(3) receptor using photonic force microscope
Author(s): Bo-Jui Chang; Chia-Fen Hsieh; Chi-Hung Lin; Sien Chi; Long Hsu
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Paper Abstract

The conformational change of integrin αIIbβ3 plays an important role in clot formation. However, the correlation between the structure and the function of integrin αIIbβ3 in interacting with its ligand is still not clear. In this report, we focus on the dynamic variation of the binding between integrin αIIbβ3 and its ligand, rhodostomin by using a photonic force microscopy (PFM). The PFM is used to trap a rhodostomin-coated bead and, then, shift it to bind a surrounding CHO αIIbβ3 cell. Meanwhile, it tracks, with a resolution of 1MHz, the Brownian fluctuations of the trapped bead. Theoretically, the smaller the amplitude of the Brownian fluctuations, the stronger the stiffness of the binding force between the rhodostomin and the CHO αIIbβ3 cell. Experimentally, a significant decrease of the Brownian fluctuations was observed during the interval between the 360th seconds and the 400th seconds after the trapped rhodostomin-coated bead contacted an integrin-expressed CHO αIIbβ3 cell. This observation reveals that it takes the rhodostomin 360 seconds to seek the correct position to bind to the integrin αIIbβ3. After 400 seconds, the rhodostomin has bound rigidly with the integrin αIIbβ3. We presume that the integrin αIIbβ3 has reached its final stage of conformational change.

Paper Details

Date Published: 18 October 2004
PDF: 8 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.559501
Show Author Affiliations
Bo-Jui Chang, National Chiao-Tung Univ. (Taiwan)
Chia-Fen Hsieh, National Yang Ming Univ. (Taiwan)
Chi-Hung Lin, National Yang Ming Univ. (Taiwan)
Sien Chi, National Chiao-Tung Univ. (Taiwan)
Long Hsu, National Chiao-Tung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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