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Proceedings Paper

Condensation of hydrodynamically stretched DNA using single-molecule fluorescence imaging and optical tweezers
Author(s): Eirini Theofanidou; Tsueu Ju Su; Jochen Arlt; David T. F. Dryden; William J. Hossack; Jason Crain; Wilson C. K. Poon
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Paper Abstract

We have observed a latency time of the order 10 s in the spermidine-induced condensation of YOYO-1-labelled DNA in flow. Higher flow speeds, longer DNA and higher salt all led to an increase in the latency time. We propose that two effects may be relevant to these observations (1) the competition between spermidine and YOYO-1 for DNA binding sites, and (2) the bending of DNA upon spermidine binding, so that flow-induced straightening of the DNA weakens spermidine's affinity for DNA. Moreover, literature data on the mechanical properties of condensed DNA chains suggest that the hydrodynamic drag forces in our experiments should indeed significantly perturb condensation kinetics.

Paper Details

Date Published: 18 October 2004
PDF: 9 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.558896
Show Author Affiliations
Eirini Theofanidou, Univ. of Edinburgh (United Kingdom)
Tsueu Ju Su, Univ. of Edinburgh (United Kingdom)
Jochen Arlt, Univ. of Edinburgh (United Kingdom)
David T. F. Dryden, Univ. of Edinburgh (United Kingdom)
William J. Hossack, Univ. of Edinburgh (United Kingdom)
Jason Crain, Univ. of Edinburgh (United Kingdom)
Wilson C. K. Poon, Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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