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Proceedings Paper

Speckle intensity correlation and optical diffusion profile in biological media
Author(s): Alex Flamholz; Patricia S. Schneider; Peter K. Wong; David H. Lieberman; Tak D. Cheung; Harriet Itoka; Troy Minott; Janie Quizhpi; Jacquelin Rodriguez
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Paper Abstract

Short-range speckle correlation techniques were used to measure the refractive index of turbid biological media. The refractive index depends on the cell content, which is about 80% water and 15% protein. The variation in water or protein content produced various small shifts in the oscillatory features of the speckle intensity spatial correlation function for correlation distances shorter than the transport mean free path. Optical diffusion profiles in transmission, and long range speckle intensity correlation techniques were used to measure the transport mean free path. The optical system was calibrated with a porous silicate slab, and live yeast was the biological system studied. It is found that the techniques employed could serve as markers for the cell's water and protein contents. Consistent results were also found for chicken tissue and a combined yeast sample. Extension to abnormal cell detection, and the application to in-situ refractive index mapping are also discussed.

Paper Details

Date Published: 2 August 2004
PDF: 9 pages
Proc. SPIE 5531, Interferometry XII: Techniques and Analysis, (2 August 2004); doi: 10.1117/12.558071
Show Author Affiliations
Alex Flamholz, CUNY/Queensborough Community College (United States)
Patricia S. Schneider, CUNY/Queensborough Community College (United States)
Peter K. Wong, CUNY/Queensborough Community College (United States)
David H. Lieberman, CUNY/Queensborough Community College (United States)
Tak D. Cheung, CUNY/Queensborough Community College (United States)
Harriet Itoka, CUNY/Queensborough Community College (United States)
Troy Minott, CUNY/Queensborough Community College (United States)
Janie Quizhpi, CUNY/Queensborough Community College (United States)
Jacquelin Rodriguez, CUNY/Queensborough Community College (United States)


Published in SPIE Proceedings Vol. 5531:
Interferometry XII: Techniques and Analysis
Katherine Creath; Joanna Schmit, Editor(s)

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