Share Email Print

Proceedings Paper

Single molecule studies of surface-induced secondary structure in a model peptide
Author(s): Douglas S. English; Joy Ann Cunningham; Sarah C. Wehri; Amy F. Petrik; Kenji Okamoto
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We have proposed using single molecule fluorescence resonant energy transfer (SM-FRET) to investigate the induction of secondary structure in model, surface-active peptides upon binding at an interface. The ability for SM-FRET to distinguish structural heterogeneity will offer a distinct advantage over traditional biophysical methods in these types of studies. Ensemble methods mask heterogeneity and only provide an average measure of secondary structural features. Because secondary structure contributes greatly to the energetics of dehydrating the amide backbone, detailed information of conformational distributions is crucial to the understanding of the thermodynamic cycle involved. Here we present results from our first efforts at using SM-FRET to study an amphipathic α-helix forming peptide immobilized at the solid-liquid interface between an aqueous solution and an octadecylsilane modified glass surface. This system serves as a model for future studies of peptide partitioning to lipid bilayers and other relevant interfaces.

Paper Details

Date Published: 14 October 2004
PDF: 6 pages
Proc. SPIE 5513, Physical Chemistry of Interfaces and Nanomaterials III, (14 October 2004); doi: 10.1117/12.557035
Show Author Affiliations
Douglas S. English, Univ. of Maryland/College Park (United States)
Joy Ann Cunningham, Univ. of Maryland/College Park (United States)
Sarah C. Wehri, Univ. of Maryland/College Park (United States)
Amy F. Petrik, Univ. of Maryland/College Park (United States)
Kenji Okamoto, Univ. of Maryland/College Park (United States)

Published in SPIE Proceedings Vol. 5513:
Physical Chemistry of Interfaces and Nanomaterials III
Gregory V. Hartland; Xiao-Yang Zhu, Editor(s)

© SPIE. Terms of Use
Back to Top