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Proceedings Paper

Spherical aberration correction for optical tweezers
Author(s): Jochen Arlt; Laurence Wilson; Eirini Theofanidou; William J. Hossack
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Paper Abstract

Typical optical tweezers setups use high numerical aperture oil-immersion objectives to trap particles suspended in an aqueous medium. When trapping deep inside a sample or out of the imaging plane the quality of the trap in such a system deteriorates due to optical aberrations caused by the refractive index mismatch at the glass-water interface. We investigate this effect experimentally by monitoring the two-photon fluorescence of trapped dye-stained polystyrene spheres. We consider the effect of the numerical aperture on the trap quality and also partly corrected the aberrations by optimising the fluorescence signal using an adaptive deformable membrane mirror.

Paper Details

Date Published: 18 October 2004
PDF: 9 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.555824
Show Author Affiliations
Jochen Arlt, Univ. of Edinburgh (United Kingdom)
Laurence Wilson, Univ. of Edinburgh (United Kingdom)
Eirini Theofanidou, Univ. of Edinburgh (United Kingdom)
William J. Hossack, Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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