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Proceedings Paper

Surface plasmon resonance imaging and versatile surface functionalization for real time comparisons of biochemical interactions
Author(s): Emmanuel Maillart; Nathalie Bassil; Pierre Lecaruyer; Michael Canva; Yves Levy
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Paper Abstract

Surface plasmon resonance imaging is an optical method that allows the real time detection of small changes in the physical properties of a dielectric medium near a metallic surface. Using proper surface functionalization and structuration, this technique can be applied to the realization of optical biochips where multiple unlabeled interactions can be monitored. More precisely, thanks to the use of an adequate optical set-up built around a gold surface realized by self assembled monolayers or electrocopolymerization, we studied DNA:DNA interactions with potential application to genetic diagnostic and DNA:protein interactions to demonstrate the ability of the system to determine simultaneously different affinity constants.

Paper Details

Date Published: 8 September 2004
PDF: 9 pages
Proc. SPIE 5461, Biophotonics New Frontier: From Genome to Proteome, (8 September 2004); doi: 10.1117/12.554998
Show Author Affiliations
Emmanuel Maillart, Lab. Charles Fabry de l'Institut d'Optique, CNRS, and Univ. Paris XI (France)
Nathalie Bassil, Lab. Charles Fabry de l'Institut d'Optique, CNRS, and Univ. Paris XI (France)
Pierre Lecaruyer, Lab. Charles Fabry de l'Institut d'Optique, CNRS, and Univ. Paris XI (France)
Michael Canva, Lab. Charles Fabry de l'Institut d'Optique, CNRS, and Univ. Paris XI (France)
Yves Levy, Lab. Charles Fabry de l'Institut d'Optique, CNRS, and Univ. Paris XI (France)


Published in SPIE Proceedings Vol. 5461:
Biophotonics New Frontier: From Genome to Proteome
Michel D. Faupel; Patrick Meyrueis, Editor(s)

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