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Proceedings Paper

Reagentless cell lysis on a PDMS CD using beads
Author(s): Jitae Kim; She-Hee Jang; Jim V. Zoval; Nancy A. Da Silva; Marc J. Madou
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Paper Abstract

Reagentless mechanical cell lysis was demonstrated on a microfluidic CD (Compact Disc) microfabricated in PDMS (Polydimethylsiloxane). The motion of beads in a lysis chamber on the CD causes disruption of mammalian (CHO-K1), bacterial (Escherichia coli), and yeast (Saccharomyces cerevisiae) cells. Interactions between beads and cells are generated in the rimming flow established inside a partially filled annular chamber in the CD rotating around a horizontal axis. To maximize bead-cell interactions, the CD was spun forward and backwards around this axis, using high acceleration for up to 7 minutes. Based on our theoretical work, we investigated the following control parameters: bead density, angular velocity, acceleration rate, and solid volume fraction, all of which influence cell lysis efficiency. Cell disruption efficiency was verified either through direct microscopic viewing or measurement of DNA concentration after cell lysing. Lysis efficiency relative to a conventional lysis protocol was also determined. In the long term, this work is geared towards CD based sample-to-answer nucleic acid analysis which will include cell lysis, DNA purification, DNA amplification, and DNA hybridization detection.

Paper Details

Date Published: 16 August 2004
PDF: 10 pages
Proc. SPIE 5455, MEMS, MOEMS, and Micromachining, (16 August 2004); doi: 10.1117/12.548757
Show Author Affiliations
Jitae Kim, Univ. of California/Irvine (United States)
She-Hee Jang, Univ. of California/Irvine (United States)
Jim V. Zoval, Univ. of California/Irvine (United States)
Nancy A. Da Silva, Univ. of California/Irvine (United States)
Marc J. Madou, Univ. of California/Irvine (United States)

Published in SPIE Proceedings Vol. 5455:
MEMS, MOEMS, and Micromachining
Hakan Urey; Ayman El-Fatatry, Editor(s)

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