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Proceedings Paper

Microscopic birefringence imaging by the local-sampling phase shifting technique
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Paper Abstract

A microscopic birefringence imaging of bio-sample is proposed. This system consists of a super luminescent diode (SLD), polarizers, a quarter-wave plate and a phase retarder. The instrument is provided to map and visualize an optical anisotropy in bio-sample. A local-sampling phase shifting technique is employed for analytic algorithm with high resolution of retardance. A Bereck compensator is used a sample for checking its accuracy. Birefringence distributions of gelatin orientation such as retardance and azimuthal direction are shown in case of applied voltage and changing temperature as its demonstration. It is possible to observe molecular orientation of bio-sample.

Paper Details

Date Published: 10 September 2004
PDF: 6 pages
Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); doi: 10.1117/12.547768
Show Author Affiliations
Mizue Ebisawa, Tokyo Univ. of Agriculture and Technology (Japan)
Yukitoshi Otani, Tokyo Univ. of Agriculture and Technology (Japan)
Norihiro Umeda, Tokyo Univ. of Agriculture and Technology (Japan)

Published in SPIE Proceedings Vol. 5462:
Biophotonics Micro- and Nano-Imaging
Dario Anselmetti, Editor(s)

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