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Proceedings Paper

Investigation on the fluorescence spectra of thioredoxin reductase from human brain
Author(s): Xiufeng Lan; Ying Liu; Yunjing Ji; Zhonghua Shen; Jian Lu; Xiao-Wu Ni; Tao Yang; Xiaosen Luo; Jiangang Liu; Lin Xu
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Paper Abstract

The technique of fluorescence spectroscopy is applied to study thioredoxin reductast (TrxR) in the cells of human brain. Experimental results show that, by the ultraviolet light irradiation (λmax=253.7nm), TrxR is able to emit two striking spectral bands of 287nm to 484nm and 560nm to 720nm. The spectral profile also consists of some narrow spike-like bands atop these two broad bands. With the concentration of TrxR decreases, the narrow spike-like bands disappear little by little. Furthermore, physical and biochemical mechanisms of fluorescence production for ultraviolet light-induced TrxR spectra and its characteristics are analyzed. The new spectroscopic information suggested in this paper may represent an effort of better understanding of the structure and conformation changes of TrxR.

Paper Details

Date Published: 8 December 2003
PDF: 6 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546563
Show Author Affiliations
Xiufeng Lan, Nanjing Univ. of Science and Technology (China)
Ying Liu, Nanjing Univ. of Science and Technology (China)
Yunjing Ji, Nanjing Univ. of Science and Technology (China)
Zhonghua Shen, Nanjing Univ. of Science and Technology (China)
Jian Lu, Nanjing Univ. of Science and Technology (China)
Xiao-Wu Ni, Nanjing Univ. of Science and Technology (China)
Tao Yang, Second Military Medical Univ. (China)
Xiaosen Luo, Second Military Medical Univ. (China)
Jiangang Liu, Second Military Medical Univ. (China)
Lin Xu, Second Military Medical Univ. (China)


Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine

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