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Proceedings Paper

Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy
Author(s): Tongsheng Chen; Yafeng Liu; Shaoqun Zeng; Qingming Luo
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Paper Abstract

The intensity-dependence of photobleaching rate with two-photon excitation (TPE) at biological imaging level has been found to be high-order (>3) relationship. This paper studied experimentally the dependence for rhodamine B (RhB) at two different concentrations under unsaturated absorption conditions. The log-log plot of photobleaching rate versus incident power at concentration of 10-2M increased with a slope of ~3.7, but that at concentration of 10-4M increased with a slope of ~2.7.

Paper Details

Date Published: 8 December 2003
PDF: 5 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546559
Show Author Affiliations
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Yafeng Liu, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Valery V. Tuchin; Min Gu; Lihong V. Wang, Editor(s)

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