Share Email Print
cover

Proceedings Paper

A method for measuring cerebral blood volume of mouse using multiphoton laser scanning microscopy
Author(s): Pascale Verant; Raphael Serduc; Jonathan A. Coles; Regine Farion; Chantal Remy; Boudewijn van der Sanden; Jean-Claude Vial
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Knowledge of the volume of blood per unit volume of brain tissue is important for understanding brain function in health and disease. We describe a novel method using two-photon laser scanning microscopy to obtain the local blood volume in the cortex of the anesthetized mouse. We infused fluorescent dyes in the circulating blood and imaged the blood vessels, including the capillaries, to a depth of 400 microns below the dura at the brain surface. Blood volume was calculated by normalizing the total fluorescence measured at each depth. This method, which dispenses with form recognition, is rapid and only weakly sensitive to background noise; it could be extended to measure the leakiness of the blood vessels.

Paper Details

Date Published: 8 September 2004
PDF: 12 pages
Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.546334
Show Author Affiliations
Pascale Verant, Lab. de Spectrometrie Physique, CNRS and Univ. Joseph Fourier (France)
Raphael Serduc, INSERM and Univ. Joseph Fourier (France)
Jonathan A. Coles, INSERM and Univ. Joseph Fourier (France)
Regine Farion, INSERM and Univ. Joseph Fourier (France)
Chantal Remy, INSERM and Univ. Joseph Fourier (France)
Boudewijn van der Sanden, INSERM and Univ. Joseph Fourier (France)
Jean-Claude Vial, Lab. de Spectrometrie Physique, CNRS and Univ. Joseph Fourier (France)


Published in SPIE Proceedings Vol. 5463:
Femtosecond Laser Applications in Biology
Sigrid Avrillier; Jean-Michel Tualle, Editor(s)

© SPIE. Terms of Use
Back to Top