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Proceedings Paper

Fluorescence correlation spectroscopy based upon two-photon excitation
Author(s): Yafeng Liu; Tongsheng Chen; Qingming Luo
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Paper Abstract

Fluorescence correlation spectroscopy (FCS) is a powerful tool for measurement of biological dynamic processes. In this studying, a two-photon excitation fluorescence correlation spectroscopy (TP-FCS) system was set up depending on a part of optical block and detector of the multi-photon excitation fluorescence microscope (MPLFM). The phenomenon "photon-burst" was observed from the TP-FCS system. Meanwhile, the diffusion coefficient of rhodamine B molecule in sucrose aqueous solution was calculated. It was proved that TP-FCS is especially suited for integration into MPEFM accordingly to yield a hybride-technology with the peculiarities of the individual technique and the advantage of mutual synergistic effects. The fusion of both techniques seems to be reasonable and desirable to reduce costs.

Paper Details

Date Published: 4 December 2003
PDF: 6 pages
Proc. SPIE 5255, Biomolecular Photonics and Multidimensional Microscopy, (4 December 2003); doi: 10.1117/12.546244
Show Author Affiliations
Yafeng Liu, Huazhong Univ. of Science and Technology (China)
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 5255:
Biomolecular Photonics and Multidimensional Microscopy
Qingming Luo; Min Gu, Editor(s)

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