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Proceedings Paper

Direct monitoring of antigen-antibody interactions by optical fiber bioprobe
Author(s): Yuxiao Yang; Kaili Xiong; Yushan Tan; Bin Liu; Yan Sun; Duanjun Chen; Hong Tan
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Paper Abstract

An optical fiber bioprobe is introduced as a biosensor to monitor the interaction of antigens and antibodies in immunoreactions. The detection is based on the theory of multiple-reflection principles in white-light interferometry. Incident light is partially reflected at the two interface of the biolayer, because of the interference resulted in the interface spectrum. The change of optical thickness can be calculated by testing the reflected spectrum phase shifting. The setup allows the consequent observation of changes in optical pathlength on a fractional nanometer scale with subsecond repetition times. The system has high sensitivity, high precision, cost effective and working on a high reliability. The testing process is nondestructive for the biolayer. The bioprobe is easy integrated as a BIAcore. The system and the experimental results on the reaction of rabbit-IgG with anti-rabbit-IgG are described in this paper.

Paper Details

Date Published: 8 December 2003
PDF: 6 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546220
Show Author Affiliations
Yuxiao Yang, Xi'an Jiaotong Univ. (China)
Kaili Xiong, Xi'an Jiaotong Univ. (China)
Yushan Tan, Xi'an Jiaotong Univ. (China)
Bin Liu, Fourth Military Medical Univ. (China)
Yan Sun, Xi'an Jiaotong Univ. (China)
Duanjun Chen, BioFiber Identification, Inc. (United States)
Hong Tan, BioFiber Identification, Inc. (United States)

Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Valery V. Tuchin; Min Gu; Lihong V. Wang, Editor(s)

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