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Proceedings Paper

Design and application of fiber optic evanescent wave biosensor
Author(s): Huijie Huang; Junhui Zhai; Yongkai Zhao; Ruifu Yang; Bingqiang Ren; Zhaogu Cheng; Longlong Du; Dunwu Lu
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Paper Abstract

A fiber-optic biosensor is developed based on the principle of evanescent wave while light propagates in optical fiber. The biosensor uses a red laser diode at 636.85 nm for exciting Cy5 fluorescent dye. Sensitivity limit of 0.01 nnmol/l is obtained from the detection of serial Cy5 solutions with various concentrations. In log-to-log plot, excellent linear response characteristic is seen in the Cy5 concentrations ranging from 0.01 nmlo/l to 100 nmol/l. And a good result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 μmol/l. All the results are comparable with those that are obtained by a commercial biochip scanner GeneTAC 1000.

Paper Details

Date Published: 8 December 2003
PDF: 7 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546217
Show Author Affiliations
Huijie Huang, Shanghai Institute of Optics and Fine Mechanics (China)
Junhui Zhai, Institute of Microbiology and Epidemiology (China)
Yongkai Zhao, Shanghai Institute of Optics and Fine Mechanics (China)
Ruifu Yang, Institute of Microbiology and Epidemiology (China)
Bingqiang Ren, Shanghai Institute of Optics and Fine Mechanics (China)
Zhaogu Cheng, Shanghai Institute of Optics and Fine Mechanics (China)
Longlong Du, Shanghai Institute of Optics and Fine Mechanics (China)
Dunwu Lu, Shanghai Institute of Optics and Fine Mechanics (China)


Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Valery V. Tuchin; Min Gu; Lihong V. Wang, Editor(s)

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