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Proceedings Paper

A high sensitivity detection method for singlet oxygen and superoxide anion and the mechanism investigation
Author(s): Min Hao; Da Xing; Juan Wang
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Paper Abstract

In this paper, a novel and highly sensitive detection method of singlet oxygen (1O2) and superoxide anion (O2-) is reported. The principle of the method is similar to that for a measurement of ATP using luciferin and luciferase. A chemiluminescence analytic reagent, 3,7-dihydro-6-{4-[2-(4’-(5-fluoreseinyl)thioureido)ethyl]phenyl}-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (FCLA), can sensitively react with 1O2 and O2- and emit photons that can be detected with a single photon counting system. The chemiluminence is significantly enhanced, approximately 20 times, when human serum albumin (HAS) is used in company with the FCLA. In order to further confirm the chemiluminance is due to the reactive oxygen species (ROS), we investigated the relationship between the intensity of the chemiluminence and the concentration of dissolved molecular oxygen, the direct transform source for 1O2 and O2- in the solution. We found that the intensity of the chemiluminence signal decreased linearly to zero, as nitrogen bursting reduced the concentration of the dissolved oxygen. The results indicate that, the FCLA-HAS combination provides a highly sensitive chemiluminence method for detecting reactive oxygen species, 1O2 and O2-.

Paper Details

Date Published: 8 December 2003
PDF: 7 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546216
Show Author Affiliations
Min Hao, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Juan Wang, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Valery V. Tuchin; Min Gu; Lihong V. Wang, Editor(s)

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