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Proceedings Paper

Designing and constructing an optical projection tomography microscopy: theoretical and practical considerations
Author(s): Yi Wang; Da Xing
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Paper Abstract

Optical projection tomography (OPT) microscopy is a new technique to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens. It can be used to rapidly map the tissue distribution of RNA and protein expression in intact embryos or organ systems. In optical projection tomography, the specimen is rotated through 360 degrees around a single axis while held in position for imaging. Light passing through specimens is focused onto a digital camera by lenses. After the images of all angles are taken. Sections of specimen are independently reconstructed using the filtered back projection algorithm. This paper presents general guidelines for designing and constructing an OPT microscopy. These guidelines are based on the difficulties encountered during the process of building the system and cover theoretical concepts, optical imaging system and reconstruction algorithm required to build the system. In addition, some suggestions are presented to improve future designs.

Paper Details

Date Published: 8 December 2003
PDF: 5 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546094
Show Author Affiliations
Yi Wang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Valery V. Tuchin; Min Gu; Lihong V. Wang, Editor(s)

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