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Proceedings Paper

Fluorometric determination of whole blood with various excitation wavelengths
Author(s): Xiufeng Lan; Shumei Gao; Changde Peng; Ying Liu; Xiao-Wu Ni
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Paper Abstract

Autofluorescence spectra from whole blood of laboratory rat are measured in this paper. The excitation lights are light emitting diode (LED), Ar+ laser, and He-Ne laser with the wavelength located at 457nm, 457.9nm, and 632.8nm respectively. The three spectral profiles are found to be substantially different, each displaying its own characteristic fluorescence bands. Ar+ laser-induced spectrum has very rich and sharp peaks. The LED-induced one has the strongest and widest fluorescence bands. And the intensity of the spectrum induced by He-Ne laser is much lower than the former two. Comparisons of those three fluorescence spectra indicate that Ar+ laser induced spectrum can show partly fine structure of blood cells. Based on the theoretical analysis, it is presented that the absorption of the fluorophores in blood cells to the wavelength of exciting light has definite selectivity, which depend on energy level structure and state of the fluorophores.

Paper Details

Date Published: 8 December 2003
PDF: 6 pages
Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); doi: 10.1117/12.546092
Show Author Affiliations
Xiufeng Lan, Nanjing Univ. of Science and Technology (China)
Shumei Gao, XuZhou Normal Univ. (China)
Changde Peng, XuZhou Normal Univ. (China)
Ying Liu, Nanjing Univ. of Science and Technology (China)
Xiao-Wu Ni, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 5254:
Third International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Valery V. Tuchin; Min Gu; Lihong V. Wang, Editor(s)

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