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Proceedings Paper

Multiphoton imaging of cardiovascular structures
Author(s): Katja Schenke-Layland; Florian Opitz; Iris Riemann; Ulrich A. Stock; Karsten Konig
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Paper Abstract

Near infrared (NIR) femtosecond laser imaging systems represent a novel and very promising diagnostic technology for non-invasive cross-sectional analysis of living biological tissues. In this study 3D multiphoton imaging with submicron resolution has been performed for non-invasive analysis of living native and tissue-engineered (TE) heart valves and blood vessels. High-resolution autofluorescence and second harmonic generation (SHG) images of collagenous structures and elastic fibers were demonstrated using multiphoton excitation at two different wavelengths. Non-invasive optical sections have been obtained without the need of staining or embedding. The quality of the resulting three-dimensional images allowed exact differentiation between collagenous structures and elastic fibers. These experimental results are very encouraging for NIR femtosecond laser scanning microscopy as a useful tool for future non-destructive monitoring and characterization of vital and intact TE cardiovascular structures.

Paper Details

Date Published: 8 September 2004
PDF: 8 pages
Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.545882
Show Author Affiliations
Katja Schenke-Layland, Friedrich-Schiller-Univ. Jena (Germany)
Florian Opitz, Friedrich-Schiller-Univ. Jena (Germany)
Iris Riemann, Fraunhofer Institute for Biomedical Technology (Germany)
Ulrich A. Stock, Friedrich-Schiller-Univ. Jena (Germany)
Karsten Konig, Fraunhofer Institute for Biomedical Technology (Germany)

Published in SPIE Proceedings Vol. 5463:
Femtosecond Laser Applications in Biology
Sigrid Avrillier; Jean-Michel Tualle, Editor(s)

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