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Proceedings Paper

The point spread function of fluorescence microscopes imaging through a layered or an inhomogeneous medium
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Paper Abstract

The optical microscope has proven to be an invaluable tool in biology, because of its unique capabilities of 3-D imaging of living specimens. However, compared to other techniques, the achievable resolution is limited. Several techniques have been proposed to improve the resolution of the fluorescence microscope. The confocal set-up is the first of them. Interference effects can also be used to sharpen up the point spread function (PSF) in 4Pi microscopy. Another approach is the so-called STimulated Emission Depletion microscopy, which has permitted to decrease the resolution down to about 100 nm in three dimensions, and below 50 nm in either the x-y plane, or along the z-axis.

Paper Details

Date Published: 10 September 2004
PDF: 10 pages
Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); doi: 10.1117/12.545792
Show Author Affiliations
Olivier Haeberle, Univ. de Haute-Alsace (France)
Bertrand Simon, Univ. de Haute-Alsace (France)

Published in SPIE Proceedings Vol. 5462:
Biophotonics Micro- and Nano-Imaging
Dario Anselmetti, Editor(s)

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