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Proceedings Paper

Nanoprocessing of DNA with femtosecond laser
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Paper Abstract

Sequence specific cutting of DNA is a standard method in molecular biology. This cutting is realized with enzymes which have a defined recognition sequence and cutting sequence. Therefore one can manipulate only sequences for which an enzyme is available. With current physical methods (AFM) any sequences can be cut, but the precise sequence specific and highly parallel cutting is not possible. Near infrared (NIR) femtosecond laser systems have been used to optically knock out genomic regions of highly condensed DNA in human chromosomes as well as of single expanded (stretched) DNA molecules. Working with 80 MHz laser pulses at 800 nm of low 2 nJ pulse energy but at high TW/cm2 light intensities, multiphoton ionization and optical breakdown (OB) resulted in highly precise material ablation with sub-100 nm cut sizes. This is far below the diffraction-limited spot size. A minimum FWHM cut size of 65 nm was achieved in the case of the nanodissection of a laser-treated stretched λ-DNA (48kb) molecule which corresponded to 200 optically knocked out bases. By the use of metal nanoparticles as energy coupling objects for fs laser radiation we expect a specific highly local destruction effect within the DNA molecule (cut). Thereby, a sequence-specific binding of DNA nanoparticle complexes along the target DNA is a fundamental condition. The effect of laser exposure on DNA and DNA-nanoparticle complexes are presented.

Paper Details

Date Published: 10 September 2004
PDF: 10 pages
Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); doi: 10.1117/12.545730
Show Author Affiliations
Karsten Koenig, Fraunhofer-Institut fur Biomedizinische Technik (Germany)
Frank Garwe, JenLab GmbH (Germany)
Andrea Czaki, Institut fur Physikalische Hochtechnologie e.V. (Germany)
Gunther Maubach, Institut fur Physikalische Hochtechnologie e.V. (Germany)
Iris Riemann, Fraunhofer-Institut fur Biomedizinische Technik (Germany)
Wolfgang Fritzsche, Institut fur Physikalische Hochtechnologie e.V. (Germany)

Published in SPIE Proceedings Vol. 5462:
Biophotonics Micro- and Nano-Imaging
Dario Anselmetti, Editor(s)

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