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Proceedings Paper

Axial localization of luminophores by partial coherence interferometry
Author(s): Nicolas Sandeau; Herve Rigneault; Pierre-Francois Lenne; Hugues Giovannini
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Paper Abstract

We propose a solution for increasing the axial resolution of confocal microscopes. In the experimental set-up described in this paper an interference phenomenon between two counterpropagating beams is used to determine the axial position of a luminophore. The optical path difference between the two waves, which is related to the position of the luminophore, is recovered thanks to a second interferometer by using partial coherence interferometry demodulation technique. The proposed solution can find applications in biology for localizing with nanometric resolution a small number of tagged species.

Paper Details

Date Published: 10 September 2004
PDF: 6 pages
Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); doi: 10.1117/12.545661
Show Author Affiliations
Nicolas Sandeau, Institut Fresnel, CNRS (France)
Herve Rigneault, Institut Fresnel, CNRS (France)
Pierre-Francois Lenne, Institut Fresnel, CNRS (France)
Hugues Giovannini, Institut Fresnel, CNRS (France)


Published in SPIE Proceedings Vol. 5462:
Biophotonics Micro- and Nano-Imaging
Dario Anselmetti, Editor(s)

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