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Proceedings Paper

Characterization of multiphoton laser scanning device optical parameters for image restoration
Author(s): Frank Fischer; Karsten Konig; Stefan Puschmann; Roger Wepf; Iris Riemann; Volker Ulrich; Peter Fischer
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Paper Abstract

Fluorescent nanobeads embedded in agarose and skin biopsies were used to optically characterize spatial and temporal resolution of multiphoton laser scanning devices (MPLSD). Optical sections based on two-photon excited bead fluorescence have been performed at various sample depths. Three-dimensional reconstruction of the image stacks allowed determination of the point spread function. Using calculated point spread functions to apply deconvolution procedures (e.g. Huygens software), the visualization and hence the interpretation of intradermal structures, such as extracellular matrix components in 150 μm tissue depth, was improved.

Paper Details

Date Published: 8 September 2004
PDF: 6 pages
Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.545604
Show Author Affiliations
Frank Fischer, Beiersdorf AG (Germany)
Karsten Konig, Fraunhofer Institute for Biomedical Technology (Germany)
Stefan Puschmann, Beiersdorf AG (Germany)
Roger Wepf, Beiersdorf AG (Germany)
Iris Riemann, Fraunhofer Institute for Biomedical Technology (Germany)
Volker Ulrich, JenLab GmbH (Germany)
Peter Fischer, JenLab GmbH (Germany)


Published in SPIE Proceedings Vol. 5463:
Femtosecond Laser Applications in Biology
Sigrid Avrillier; Jean-Michel Tualle, Editor(s)

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