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Proceedings Paper

Vibrational imaging by coherent anti-stokes Raman scattering (CARS) microscopy
Author(s): Nadia Djaker; Pierre-Francois Lenne; Herve Rigneault
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Paper Abstract

An approach for the chemical imaging of molecular systems is presented, based on coherent anti-Stokes Raman scattering microscopy/spectroscopy (CARS). This technique permits to map selectively molecular species, by using vibrational properties of their chemical bounds. This offers a powerful optical tool with high sensitivity, high spatial resolution and three dimensional sectioning capability, without using fluorophores that are prone to photobleaching. CARS does not need confocal detection since the contrast mechanism results from a non-linear process (four wave mixing), that is generated only in epidetection configuration at the microscope objective focal point. We present the first results of our experiment set up on polystyrene beads.

Paper Details

Date Published: 8 September 2004
PDF: 7 pages
Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.544999
Show Author Affiliations
Nadia Djaker, Institut Fresnel, CNRS, EGIM, and Univ. Aix-Marseille (France)
Pierre-Francois Lenne, Institut Fresnel, CNRS, EGIM, and Univ. Aix-Marseille (France)
Herve Rigneault, Institut Fresnel, CNRS, EGIM, and Univ. Aix-Marseille (France)

Published in SPIE Proceedings Vol. 5463:
Femtosecond Laser Applications in Biology
Sigrid Avrillier; Jean-Michel Tualle, Editor(s)

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