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Proceedings Paper

An in vitro study of the effects of low-level laser radiation on human blood
Author(s): Dan G. Siposan; Adalbert Lukacs
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Paper Abstract

In the last time the study of the effects of LLLR on the blood is considered to be a subject of great importance in elucidating the mechanisms of action between LLLR and biologic tissues. Different methods of therapy by blood irradiation have been developed and used in clinical purposes with benefic effects. This study investigates some in vitro effects of LLLR on some selected rheologic indices of human blood. After establishing whether or not damaging effects could appear due to laser irradiation of the blood, we tried to find a new method for rejuvenating the blood preserved in MacoPharma-type bags. Blood samples were obtained from adult regular donors (volunteers). HeNe laser and laser diodes were used as radiation source, in a wide range of wavelengths, power densities, doses and other parameters of irradiation protocol. In the first series of experiments we established that LLLR does not alter the fresh blood from healthy donors, for doses between 0 and 10 J/cm3 and power densities between 30 and 180 mW/cm3. In the second series of experiments we established that LLLR does have, in some specific conditions, a revitalizing effect on the erythrocytes in preserved blood. We concluded that laser irradiation of the preserved blood, following a selected protocol of irradiation, could be used as a new method to improve the performances of preservation: prolonging the period of storage and blood rejuvenation before transfusion.

Paper Details

Date Published: 12 December 2003
PDF: 12 pages
Proc. SPIE 5287, Laser Florence 2002: A Window on the Laser Medicine World, (12 December 2003); doi: 10.1117/12.544890
Show Author Affiliations
Dan G. Siposan, Technical Military Academy (Romania)
Adalbert Lukacs, Ctr. of Transfusion Hematology of the Army (Romania)


Published in SPIE Proceedings Vol. 5287:
Laser Florence 2002: A Window on the Laser Medicine World
Leonardo Longo; Alfons G. Hofstetter; Mihail-Lucien Pascu; Wilhelm R. A. Waidelich, Editor(s)

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