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Proceedings Paper

Multiphoton imaging of excised normal skin and keloid scar: preliminary investigations
Author(s): Michael B. Brewer; Alvin T. Yeh; Behrooz Torkian; Chung-Ho Sun; Bruce J. Tromberg; Brian Jet-Fei Wong
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Paper Abstract

Wound healing is a physiologic process that acts to repair disruptions in the continuity of tissue caused by injury or surgical incision. Keloids and hypertrophic scars are forms of aberrant wound healing, which are characterized by the overproduction of collagen, resulting in an excessive amount of scar tissue. Keloid tumors, by definition, grow outside the boundary of the original tissue damage. Multiphoton microscopy (MPM) is an imaging technique which allows imaging of living specimens, without the use of fixation or stains. Images of collagen fibers are produced by the second harmonic signal intensity generated by endogenous fluorescence through excitation by infrared laser light. A postauricular keloid tumor was excised from a patient. The tissue was dissected, and a portion was imaged using MPM. Normal skin tissue was isolated from a patient undergoing a facelift. A portion of this tissue was also dissected and imaged using MPM. MPM images were taken using a 63X water immersion objective lens on a two-photon microscope and a titanium-sapphire laser. Images were taken beginning at the surface of the tissue and moving in at intervals of 200 nm to a final depth of 30 μm. The two-photon images were used to reconstruct three-dimensional representations of the collagen matrix within the tissues, which are readily contrasted. Density of the collagen within each tissue was also ascertained using depth dependant decay of the image intensity. Multiphoton imaging was successfully used to image the collagen matrix of normal skin and a keloid scar, demonstrating differences in their microstructures.

Paper Details

Date Published: 13 July 2004
PDF: 5 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.543540
Show Author Affiliations
Michael B. Brewer, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Alvin T. Yeh, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Behrooz Torkian, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Chung-Ho Sun, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Brian Jet-Fei Wong, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)


Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Brian Jet-Fei Wong; Nikiforos Kollias; Kenton W. Gregory; Henry Hirschberg; Reza S. Malek; Abraham Katzir; David S. Robinson; Kenneth Eugene Bartels; Eugene A. Trowers; Werner T.W. de Riese; Lawrence S. Bass; Lloyd P. Tate; Steen J. Madsen; Keith D. Paulsen; Karen M. McNally-Heintzelman, Editor(s)

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