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Proceedings Paper

In vivo and intracellular sensing and imaging of free zinc ion
Author(s): Rebecca A. Bozym; Hui-Hui Zeng; Michele Cramer; Andrea Stoddard; Carol A. Fierke; Richard B. Thompson
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Paper Abstract

We describe new methods for the study of zinc in biological specimens. Intracellular free zinc was determined at levels down to picomolar using an excitation ratiometric fluorescence-based biosensing approach using a carbonic anhydrase variant as transducer. A new fiber optic sensor suitable for in vivo use is also described using laser excitation and an emission ratiometric approach; the zinc concentration range of sensor response can be selected to fit the application.

Paper Details

Date Published: 1 July 2004
PDF: 5 pages
Proc. SPIE 5318, Advanced Biomedical and Clinical Diagnostic Systems II, (1 July 2004); doi: 10.1117/12.542329
Show Author Affiliations
Rebecca A. Bozym, Univ. of Maryland School of Medicine (United States)
Hui-Hui Zeng, Univ. of Maryland School of Medicine (United States)
Michele Cramer, Univ. of Maryland School of Medicine (United States)
Andrea Stoddard, Univ. of Michigan (United States)
Carol A. Fierke, Univ. of Michigan (United States)
Richard B. Thompson, Univ. of Maryland School of Medicine (United States)


Published in SPIE Proceedings Vol. 5318:
Advanced Biomedical and Clinical Diagnostic Systems II
Gerald E. Cohn; Warren S. Grundfest; David A. Benaron; Tuan Vo-Dinh, Editor(s)

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