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Proceedings Paper

Second harmonic imaging of plant polysaccharides
Author(s): Guy C. Cox; Jose Feijo
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Paper Abstract

The application of second harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterisation of biologically and commercially important plant polysaccharides. We have found that imaging of cellulose requires rather high laser powers which are above optimal values for live cell imaging. Starch, however is easily imaged by the technique at laser fluences compatible with extended cell viability. This also has useful applications in imaging plant-derived starchy food products. Lignin in plant cell walls shows a strong 3-photon excited fluorescence which may be enhanced by resonance effects.

Paper Details

Date Published: 21 June 2004
PDF: 8 pages
Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); doi: 10.1117/12.540014
Show Author Affiliations
Guy C. Cox, Univ. of Sydney (Australia)
Instituto Gulbenkian de Ciencia (Portugal)
Jose Feijo, Instituto Gulbenkian de Ciencia (Portugal)

Published in SPIE Proceedings Vol. 5323:
Multiphoton Microscopy in the Biomedical Sciences IV
Ammasi Periasamy; Peter T. C. So, Editor(s)

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