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Proceedings Paper

Demonstration of optical microfluidics
Author(s): Kenneth T. Kotz; Konstantinos S. Kalogerakis; Kyle A. Noble; Sarah E. Smith; Gregory W. Faris
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Paper Abstract

We demonstrate a novel method for the control of small droplets using laser-based heating. Temperature dependent interfacial surface tensions were the primary force used to move droplets. With this approach, ~1.7 μL to 14 pL droplets were moved on a bare, unmodified polystyrene surface, at speeds of up to 3 mm/s. Upon contact, droplets spontaneously fused and rapidly mixed within 33 ms. We performed an optical absorption-based protein assay using horseradish peroxidase and a chromogenic substrate (ABTS), and readily detected as little as ~125 attomoles of reacting enzyme.

Paper Details

Date Published: 22 June 2004
PDF: 6 pages
Proc. SPIE 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, (22 June 2004); doi: 10.1117/12.538890
Show Author Affiliations
Kenneth T. Kotz, SRI International (United States)
Konstantinos S. Kalogerakis, SRI International (United States)
Kyle A. Noble, SRI International (United States)
Sarah E. Smith, SRI International (United States)
Gregory W. Faris, SRI International (United States)

Published in SPIE Proceedings Vol. 5328:
Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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