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Proceedings Paper

In vivo multiphoton microscopy of deep tissue with gradient index lenses
Author(s): Michael J. Levene; Daniel A. Dombeck; Rebecca M. Williams; Jesse Skoch; Gregory A. Hickey; Karl A. Kasischke; Raymond P. Molloy; Martin Ingelsson; Edward A. Stern; Jochen Klucken; Brian J. Bacskai; Warren R. Zipfel; Bradley T. Hyman; Watt W. Webb
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Paper Abstract

Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.

Paper Details

Date Published: 21 June 2004
PDF: 6 pages
Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); doi: 10.1117/12.537285
Show Author Affiliations
Michael J. Levene, Cornell Univ. (United States)
Daniel A. Dombeck, Cornell Univ. (United States)
Rebecca M. Williams, Cornell Univ. (United States)
Jesse Skoch, Massachusetts General Hospital (United States)
Gregory A. Hickey, Massachusetts General Hospital (United States)
Karl A. Kasischke, Cornell Univ. (United States)
Raymond P. Molloy, Cornell Univ. (United States)
Martin Ingelsson, Massachusetts General Hospital (United States)
Edward A. Stern, Massachusetts General Hospital (United States)
Jochen Klucken, Massachusetts General Hospital (United States)
Brian J. Bacskai, Massachusetts General Hospital (United States)
Warren R. Zipfel, Cornell Univ. (United States)
Bradley T. Hyman, Massachusetts General Hospital (United States)
Watt W. Webb, Cornell Univ. (United States)


Published in SPIE Proceedings Vol. 5323:
Multiphoton Microscopy in the Biomedical Sciences IV
Ammasi Periasamy; Peter T. C. So, Editor(s)

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