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Proceedings Paper

Visualization of tumor-induced VEGF expression using in vivo bioluminescence
Author(s): Shannon Faley; Cornelia Crooke; Keiko Takahashi; Takamune Takahashi; E. Duco Jansen
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Paper Abstract

Vascular endothelial growth factor (VEGF) is one of the most potent mediators of both physiologic and pathologic angiogenesis. Normal physiologic induction of VEGF occurs during periods of extreme growth, wound healing, as well as immune inflammatory response. Pathologically, however, VEGF is largely responsible for tumor induced angiogenesis and cell survival. Traditional methods of VEGF expression analysis involve either in vitro studies, or highly invasive in vivo methods. We have developed a unique transgenic mouse model (VGL) that possesses a truncated human VEGF promoter attached to a GFP-Luciferase fusion protein. Incorporating this model with both spontaneous and orthotopically injected tumors allow VEGF promoter activity to be visualized in vivo by luciferase luminescence in response to tumor growth non-invasively and over time. By also utilizing bioluminescent tumor cells, we were able to generate models that identify host, tumor, or combined VEGF promoter activity. Results indicate that tumor tissue is responsible for the majority of VEGF promoter activity during tumor growth. Additional studies into the mechanism by which tumor cells initiate VEGF production will yield much needed insight into tumor survival. In conclusion, we have shown that the VGL bioluminescent mouse model is indeed capable of yielding compelling information on host-tumor interactions.

Paper Details

Date Published: 14 June 2004
PDF: 9 pages
Proc. SPIE 5329, Genetically Engineered and Optical Probes for Biomedical Applications II, (14 June 2004); doi: 10.1117/12.536430
Show Author Affiliations
Shannon Faley, Vanderbilt Univ. (United States)
Cornelia Crooke, Vanderbilt Univ. Medical Ctr. (United States)
Keiko Takahashi, Vanderbilt Univ. Medical Ctr. (United States)
Takamune Takahashi, Vanderbilt Univ. Medical Ctr. (United States)
E. Duco Jansen, Vanderbilt Univ. (United States)


Published in SPIE Proceedings Vol. 5329:
Genetically Engineered and Optical Probes for Biomedical Applications II
Alexander P. Savitsky; Darryl J. Bornhop; Ramesh Raghavachari; Samuel I. Achilefu, Editor(s)

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