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Proceedings Paper

Development of a percutaneous optical imaging system for tracking vascular gene expression: a feasibility study using human tissuelike phantoms
Author(s): Sourav Kumar Kar; Ananda Kumar; Xiaoming Yang
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Paper Abstract

Noninvasive tracking of vascular gene delivery and expression forms an important part of successfully implementing vascular gene therapy methods for the treatment of atherosclerosis and various cardiovascular disorders. While ultrasound and MR imaging have shown promise in the monitoring of gene delivery to the vasculatures, optical imaging has shown promise for tracking gene expression. Optical imaging using bioreporter genes like Green Fluorescent Protein (GFP), Red Fluorescent Protein (RFP) and Luciferase to track and localize the therapeutic gene have helped provide an in vivo detection method of the process. The usage of GFP and RFP entails the detection of the fluorescent signal emitted by them on excitation with light of appropriate wavelength. We have developed a novel percutaneous optical imaging system that may be used for in vivo tracking vascular fluorescent gene expression in deep-seated vessels. It is based on the detection of the fluorescent signal emitted from GFP tagged cells. This phantom study was carried out to investigate the performance of the optical imaging system and gain insights into its performance record and study improvisation possibilities.

Paper Details

Date Published: 5 May 2004
PDF: 8 pages
Proc. SPIE 5367, Medical Imaging 2004: Visualization, Image-Guided Procedures, and Display, (5 May 2004); doi: 10.1117/12.535598
Show Author Affiliations
Sourav Kumar Kar, Johns Hopkins Univ. (United States)
Ananda Kumar, Johns Hopkins Univ. (United States)
Xiaoming Yang, Johns Hopkins Univ. (United States)


Published in SPIE Proceedings Vol. 5367:
Medical Imaging 2004: Visualization, Image-Guided Procedures, and Display
Robert L. Galloway, Editor(s)

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