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Proceedings Paper

Velocity imaging in microfluidic devices using fluorescence correlation spectroscopy
Author(s): Kalyan K Kuricheti; Volker Buschmann; Paul Brister; Kenneth D Weston
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Paper Abstract

In this paper we present and demonstrate a technique for mapping fluid flow rates in microfluidic systems with sub-micron resolution using confocal microscopy in conjunction with fluorescence correlation spectroscopy. Velocity profiles and velocity images of the fluid within poly(dimethylsiloxane)-glass microchannels are presented and analyzed. Flow velocities ranging from a few μm/s to a few cm/s can be recorded using nanometer-scale fluorescent polymer spheres as fluid motion tracers. The method is applied to mapping the hydrodynamic flow velocity in complex geometries. This is, to our knowledge, the first report of FCS for producing 2-dimensional velocity maps.

Paper Details

Date Published: 23 December 2003
PDF: 12 pages
Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003); doi: 10.1117/12.532747
Show Author Affiliations
Kalyan K Kuricheti, Florida State Univ. (United States)
Volker Buschmann, Florida State Univ. (United States)
Paul Brister, Florida State Univ. (United States)
Kenneth D Weston, Florida State Univ. (United States)

Published in SPIE Proceedings Vol. 5345:
Microfluidics, BioMEMS, and Medical Microsystems II
Peter Woias; Ian Papautsky, Editor(s)

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