Share Email Print
cover

Proceedings Paper

Nanosqueezed light for probing mitochondria and calcium-induced membrane swelling for study of neuroprotectants
Author(s): Paul L. Gourley; P. Chen; R. G. Copeland; Judy Kay Hendricks; Anthony E. McDonald; M. E. Keep; J. R. Karlsson
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We report a new bioMEMs nanolaser technique for measuring characteristics of small organelles. We have initially applied the method to study mitochondria, a very small (500nm to 1um) organelle containing the respiration apparatus for animal cells. Because the mitochondria are so tiny, it has been difficult to study them using standard light microscope or flow cytometry techniques. We employ a recently discovered a nano-optical transduction method for high-speed analysis of submicron organelles. This ultrasensitive detection of submicron particles uses nano-squeezing of light into photon modes imposed by the ultrasmall organelle dimensions in a submicron laser cavity. In this paper, we report measurements of mitochondria spectra under normal conditions and under high calcium ion gradient conditions that upset membrane homeostasis and lead to organelle swelling and lysis, similar to that observed in the diseased state. The measured spectra are compared with our calculations of the electromagnetic modes in normal and distended mitochondria using multiphysics finite element methods.

Paper Details

Date Published: 23 December 2003
PDF: 10 pages
Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003); doi: 10.1117/12.532188
Show Author Affiliations
Paul L. Gourley, Sandia National Labs. (United States)
P. Chen, Sandia National Labs. (United States)
R. G. Copeland, Sandia National Labs. (United States)
Judy Kay Hendricks, Sandia National Labs. (United States)
Anthony E. McDonald, Sandia National Labs. (United States)
M. E. Keep, Univ. of New Mexico (United States)
J. R. Karlsson, Univ. of New Mexico (United States)


Published in SPIE Proceedings Vol. 5345:
Microfluidics, BioMEMS, and Medical Microsystems II
Peter Woias; Ian Papautsky, Editor(s)

© SPIE. Terms of Use
Back to Top